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CartBovine CD3 Monoclonal Antibody (clone MM1A) - Serum-free Media
Background
The CD3 T-cell co-receptor is a protein complex and is composed of four distinct chains. In mammals, the complex contains a CD3γ chain, a CD3δ chain, and two CD3ε chains. These chains associate with the T-cell receptor (TCR) and the ζ-chain to generate an activation signal in T lymphocytes. The TCR, ζ-chain, and CD3 molecules together comprise the TCR complex.
Specifications
Clone - MM1A
Isotype - IgG1
Host - Mouse
Known Reactivity - Bison, Bovine, Water Buffalo
Purification - Anti-bovine CD3 monoclonal antibody, clone MM1A, was produced in cell culture medium containing a supplement that replaces serum, clarified by filtration through a 0.2 micrometer filter.
Application
Flow Cytometry - Flow cytometric profile obtained with bovine leukocytes.
Expansion, isolation and first characterization of bovine Th17 lymphocytes.
Cunha P, Vern YL, Gitton C, Germon P, Foucras G, Rainard P.
Sci Rep. 2019 Nov 6;9(1):16115. doi: 10.1038/s41598-019-52562-2.
Applications: Isolation of CD4+ cells; Measurement of CD45RO by flow cytometric analysis; Staining of IL-17A
Abstract
Interleukin 17A-producing T helper cells (Th17) are CD4+ T cells that are crucial to immunity to extracellular bacteria. The roles of these cells in the bovine species are poorly defined, because the characterization of bovine Th17 cells lags behind for want of straightforward cultivation and isolation procedures. We have developed procedures to differentiate, expand, and isolate bovine Th17 cells from circulating CD4+ T cells of adult cows. Using polyclonal stimulation with antibodies to CD3 and CD28, we expanded IL-17A-positive CD4+ T cells in a serum-free cell culture medium supplemented with TGF-β1, IL-6 and IL-2. Populations of CD4+ T cells producing IL-17A or IFN-γ or both cytokines were obtained. Isolation of IL-17A-secreting CD4+ T cells was performed by labelling surface IL-17A, followed by flow cytometry cell sorting. The sorted Th17 cells were restimulated and could be expanded for several weeks. These cells were further characterized by cytokine profiling at transcriptomic and protein levels. They produced high amounts of IL-17A and IL-17F, and moderate amounts of IL-22 and IFN-γ. The techniques developed will be useful to characterize the phenotypic and functional properties of bovine Th17 cells.
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Kingfisher Biotech, Inc.
1000 Westgate Drive
Suite 123
Saint Paul, MN 55114
USA
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Kingfisher Biotech brand products are warranted by Kingfisher Biotech, Inc. to meet stated product specifications and to conform to label descriptions when used, handled and stored according to instructions. Unless otherwise stated, this warranty is limited to one year from date of sale. Kingfisher Biotech’s sole liability for the product is limited to replacement of the product or refund of the purchase price. Kingfisher Biotech brand products are supplied for research applications. They are not intended for medicinal, diagnostic or therapeutic use. The products may not be resold, modified for resale or used to manufacture commercial products without prior written approval from Kingfisher Biotech.
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