Component | Usage | Quantity | Catalog # |
Anti-Bovine CTLA-4 Polyclonal Antibody | Capture Antibody | 100 µg | KP1972B-100 |
Biotinylated Anti-Bovine CTLA-4 Polyclonal Antibody | Detection Antibody | 50 µg | KPB1973B-050 |
Bovine CTLA-4 Recombinant Protein | Standard | 5 µg | RP1411B-005 |
The Bovine CTLA-4 Do-It-Yourself ELISA contains capture antibody, protein standard, and detection antibody for development of a Bovine CTLA-4 ELISA. The antibodies have been determined to function in an ELISA with the standard provided. Optimal buffers, concentrations, incubation times, incubation temperatures, and methods for the ELISA have not been determined. A working knowledge of ELISA is strongly recommended. The quantities of components provided are not matched. Components may also be purchased separately.
The Bovine CTLA-4 Do-It-Yourself ELISA can also be used to measure Bison, Carabao, Wild Yak, Water Buffalo, and Zebu CTLA-4.
For additional tips and techniques to ensure a successful ELISA, check out our ELISA Technical Guide.
CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses. It is a member of the Ig gene superfamily and along with its homologue, CD28, is a B7 binding protein. CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. Alternatively spliced mRNA generates a soluble form, called sCTLA-4. Whereas low levels of sCTLA-4 are detected in normal human serum, increased/high serum levels are observed in several autoimmune diseases.
Alternate Names - CTLA4, ALPS5, CD, CD152, CELIAC3, CTLA-4, GRD4, GSE, IDDM12, cytotoxic T-lymphocyte associated protein 4
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