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CartSwine IFN gamma Polyclonal Antibody - Biotinylated
Reactivity - ELISA
Bovine IFN-γ - Moderate
Canine IFN-γ - None
Caprine IFN-γ - Moderate
Chicken IFN-γ - None
Cynomolgus Monkey IFN-γ - Weak
Dolphin IFN-γ - Moderate
Equine IFN-γ - Weak
Feline IFN-γ - Weak
Human IFN-γ - Moderate
Mouse IFN-γ - Weak
Ovine IFN-γ - Moderate
Rabbit IFN-γ - Moderate
Swine IFN-γ - Strong
Zebrafish IFN-γ1-1 - None
Zebrafish IFN-γ1-2 - None
Swine IFN-γ ELISA Data
Changes in Glomerular Filtration Rate After Renal Revascularization Correlate With Microvascular Hemodynamics and Inflammation in Swine Renal Artery Stenosis.
Eirin A, Ebrahimi B, Zhang X, Zhu XY, Tang H, Crane JA, Lerman A, Textor SC, Lerman LO.
Circ Cardiovasc Interv. 2012 Oct 9.
Applications: Measurement of Swine MCP-1 (CCL2), IFN gamma, and IL-17A in plasma by ELISA.
BACKGROUND:
The selection of patients with renal artery stenosis (RAS) likely to improve glomerular filtration rate (GFR) after percutaneous transluminal renal angioplasty is difficult. We examined basal hemodynamic and inflammatory factors linked to improved stenotic kidney (STK) function after percutaneous transluminal renal angioplasty in swine RAS.
METHODS AND RESULTS:
Fifteen pigs after 6 weeks of hemodynamically significant RAS were studied before and 4 weeks after technically successful percutaneous transluminal renal angioplasty+stenting. STK and contralateral kidney hemodynamics and function were evaluated by multidetector computed-tomography before and after acetylcholine challenge. Single-kidney deoxyhemoglobin (R2*, reciprocal to blood relaxation) and energy-dependent tubular function were assessed using blood-oxygen-level-dependent magnetic resonance imaging before and after furosemide. Baseline renal vein and inferior vena cava levels of inflammatory markers were measured and their gradient and net release calculated. Baseline parameters were compared with normal (n=7) and sham-RAS (n=7) pigs and correlated with the change in STK-GFR after revascularization (ΔGFR). Four weeks after percutaneous transluminal, renal angioplasty blood pressure was normalized in all animals, but STK-GFR improved in 10 of 15 (ΔGFR =+22.0±8.5 mL/min). ΔGFR correlated inversely with basal STK-GFR, renal release of inflammatory markers, and medullary R2* response to furosemide, but directly with GFR response to acetylcholine. Basal contralateral kidney GFR correlated directly with ΔGFR.
CONCLUSIONS:
Low basal STK-GFR with preserved response to acetylcholine may predict benefit from revascularization in RAS, whereas renal inflammation and robust STK-R2* responses to furosemide (possibly reflecting avid tubular oxygen consumption) are associated with less favorable outcomes. These tools may be useful for identification of patients likely to improve renal function after revascularization.
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